RTS DnaK Supplement
- Biotechrabbit
- BR1401601
- Na skladě
- 9 100,00Kč
-
8 925,00Kč
- OR -
Tento výrobek má minimální množství 1
Dopravné a balné
Transportní
podmínky
cena se připočítává k položkám v objednávce
mražené na suchém ledu (dry ice) 890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma
Features
- Enhance solubility of proteins expressed with RTS E. coli kits
- Improve functional activity of proteins expressed with RTS E. coli kit
- No substrate limit regarding protein size
- Easily combine with RTS 100 and 500 E. coli platforms
Applications
- E. coli Chaperone to enhance correct folding and increase solubility even of large proteins
Catalog #
Size
Package Content
BR1401601
5 rxn of 1 ml
Mix of DnaK, DnaJ and GrpE
Energy Mix (contains ATP)- The Rapid Translation System (RTS) is a flexible and scalable tool for cell-free protein expression. It is an “open” system, meaning that the reaction conditions can easily be adapted in a protein-specific manner by adding co-factors, ligands, detergents, or chaperones to the reaction mixture. DnaK (heat shock protein 70, hsp 70), in combination with DnaJ (hsp 40) and GrpE, is one of the key chaperone systems in E. coli. The RTS DnaK Supplement can be used to increase the amount of correctly folded and/or soluble proteins expressed in RTS.
In contrast to the GroE chaperone system, the applicability of DnaK is not limited by the size of the target proteins it binds to. It can also serve as an aggregation inhibitor which binds unfolded protein, or transfers the protein to other chaperones such as the GroE or the Hsp90 systems.
The following cell-free protein expression systems can be used with the RTS DnaK Supplement: RTS 100 E. coli HY Kit, RTS 500, ProteoMaster E. coli HY Kit, and RTS 500 E. coli HY Kit.